Journal: PLoS ONE

Article Title: Water Extract from the Leaves of Withania somnifera Protect RA Differentiated C6 and IMR-32 Cells against Glutamate-Induced Excitotoxicity

doi: 10.1371/journal.pone.0037080

Figure Lengend Snippet: Cell viability and toxicity of various concentrations of glutamate was assayed by MTT and LDH assays in RA differentiated C6 (b,c) and IMR-32 (g,h) cells. (d) and (i) histograms represents the relative percentage viability of glutamate and ASH-WEX treated C6 and IMR-32 cells, respectively, as compared to the control cells. (e) and (j) histograms represents the relative LDH activity when the control and ASH-WEX pretreated cells were exposed to different glutamate concentrations. “*” represents the statistical significant difference between all the treatment groups (glutamate alone or glutamate + ASH-WEX groups) with respect to control group. “#” represents the statistical difference between “glutamate + ASH-WEX” treated groups with their respective “glutamate” treatment groups. “*” and “#” = p<0.05.

Article Snippet: C6 (rat glioma) and IMR-32 (human neuroblastoma) cells were purchased from National Centre for Cell Science (Pune, India).

Techniques: Control, Activity Assay

Journal: PLoS ONE

Article Title: Water Extract from the Leaves of Withania somnifera Protect RA Differentiated C6 and IMR-32 Cells against Glutamate-Induced Excitotoxicity

doi: 10.1371/journal.pone.0037080

Figure Lengend Snippet: RT-PCR results for GFAP and NF200 mRNA in C6 (b) and IMR-32 (f) cells, respectively and their relative densitometry analysis was represented by histograms. The expression of GFAP in C6 (c) and NF200 in IMR-32 (g) cells was analysed by immunocytostaining and relative intensity was plotted as histogram as analysed by Image pro-plus software. “*” represents the statistical significant difference between all the treatment groups (ASH-WEX alone, glutamate alone or glutamate + ASH-WEX groups) with respect to control group. “#” represents the statistical difference between “glutamate + ASH-WEX” treated groups with their respective “glutamate” treatment groups. “*” and “#” = p<0.05.

Article Snippet: C6 (rat glioma) and IMR-32 (human neuroblastoma) cells were purchased from National Centre for Cell Science (Pune, India).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Software, Control

Journal: PLoS ONE

Article Title: Water Extract from the Leaves of Withania somnifera Protect RA Differentiated C6 and IMR-32 Cells against Glutamate-Induced Excitotoxicity

doi: 10.1371/journal.pone.0037080

Figure Lengend Snippet: RT-PCR results for HSP70 mRNA in C6 (b) and IMR-32 (f) cells, respectively and their relative densitometry analysis was represented by histograms. The expression of HSP70 in C6 (c) and IMR-32 (g) cells was analysed by immunocytostaining and relative intensity was plotted as histogram as analysed by Image pro-plus software. “*” represents the statistical significant difference between all the treatment groups (ASH-WEX alone, glutamate alone or glutamate + ASH-WEX groups) with respect to control group. “#” represents the statistical difference between “glutamate + ASH-WEX” treated groups with their respective “glutamate” treatment groups. “*” and “#” = p<0.05.

Article Snippet: C6 (rat glioma) and IMR-32 (human neuroblastoma) cells were purchased from National Centre for Cell Science (Pune, India).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Software, Control

Journal: PLoS ONE

Article Title: Water Extract from the Leaves of Withania somnifera Protect RA Differentiated C6 and IMR-32 Cells against Glutamate-Induced Excitotoxicity

doi: 10.1371/journal.pone.0037080

Figure Lengend Snippet: RT-PCR results for NCAM mRNA in C6 (b) and IMR-32 (e) cells, respectively and their relative densometery analysis was represented by histograms. The expression of NCAM in C6 (c) and IMR-32 (f) cells was analysed by immunostaining. “*” represents the statistical significant difference between all the treatment groups (ASH-WEX alone, glutamate alone or glutamate + ASH-WEX groups) with respect to control group. “#” represents the statistical difference between “glutamate + ASH-WEX” treated groups with their respective “glutamate” treatment groups. “*” and “#” = p<0.05.

Article Snippet: C6 (rat glioma) and IMR-32 (human neuroblastoma) cells were purchased from National Centre for Cell Science (Pune, India).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Control

Journal: PLoS ONE

Article Title: Water Extract from the Leaves of Withania somnifera Protect RA Differentiated C6 and IMR-32 Cells against Glutamate-Induced Excitotoxicity

doi: 10.1371/journal.pone.0037080

Figure Lengend Snippet: RT-PCR results for PST mRNA in C6 (b) and IMR-32 (e) cells, respectively and their relative densometery analysis was represented by histograms. The expression of PSA-NCAM in C6 (c) and IMR-32 (f) cells was analysed by immunostaining. “*” represents the statistical significant difference between all the treatment groups (ASH-WEX alone, glutamate alone or glutamate + ASH-WEX groups) with respect to control group. “#” represents the statistical difference between “glutamate + ASH-WEX” treated groups with their respective “glutamate” treatment groups. “*” and “#” = p<0.05.

Article Snippet: C6 (rat glioma) and IMR-32 (human neuroblastoma) cells were purchased from National Centre for Cell Science (Pune, India).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Control